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Our project consists of take fruits containing latex to study it and their laticifer cells. For this we used a green papaya, a jackfruit, a breadfruit, a sapote and a caimito.

The goal of this is to know if there are differences of rubber in some fruits than the rubber used to make condoms or chewing gum.

For the first experience we used the microscope with normal light and taken some photos with an app installed in the computer and the professional camera connected to it. We had to grab 20 μL of latex of each fruit and put it in microscope slides, we placed it in the stage and we choose an ocular adapted

Then, we have succeeded to seen the particles of each fruit but they were excited and they're moved everywhere, that's the brownian motion, is the random motion of particles suspended in a fluid (a liquid or a gas) resulting from their collision with the fast-moving molecules in the fluid.

After that, like we know, biological samples often need to be solidified to allow fine sectioning. Thin slices improve the access of dyes, probes, and antibodies and reduce the overlay of different cells layers. For light microscopy, paraffin wax is the most frequently used hard matrix for cutting.Here we used a embedding cassette, they are used in conjunction with conventional stainless steel covers. High-density material helps prevent floating and is resistant.


We have to heat paraffin to liquify, put the sample (in this case the piece of fruit) in the cassette, we pour the paraffin inside, then put the steel cover and finally we can put it in the fridge. We wait around 2 days for the solidification of paraffin.

Paraffin sections for light microscopy are typically 5 μm thick but here we used 8 μm. Paraffin wax does provide a sufficiently hard matrix for cutting thinner slices needed for epifluorescence microscopy. We does that with a microtome, is it, we a tool used to cut extremely thin slices of material, known as sections. So, we can see the laticifers cells inside fruits with the epifluorescent microscope.

For use this microscope, we need to used the mercury lamp, and like his name say need to have a fluorescent substance. In this case we used the fluorophore (A fluorophore is a fluorescent chemical compound that can re-emit light upon light excitation) Nile Red, is used to localize and quantitate lipids, particularly neutral lipid droplets within cells, So, we can say this chemical substance gets into the hydrophobic part of the cell for just can see the laticifer cells and not the water who are inside fruits.